Quikchange primer design

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Primer Design: DNA-based · Protein-based · Primer Characterization · Documentation ... QuikChange Site-Directed Mutagenesis Kit by Stratagene, ExSite Site- ...

QuikChange Primer Design

QuikChange Primer Design. By hand: The mutagenic oligonucleotide primers for use in this protocol must be designed individually according to the desired ...

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First the mutagenic primers are annealed to denatured template DNA. (Note that all oligonucleotides are designed to bind the same strand of the template DNA.) ...

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Design primers for generating deletions, base substitutions, or additions using a flexible, accurate system.

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1.3: Designing Primers for Site-Directed Mutagenesis

(2) Quickchange provides less significant amplification of the target DNA sequence than conventional PCR. Therefore, extra care should to be ...

NEBaseChanger can design primers specific to the mutagenesis experiment you are performing and calculate a recommended custom annealing temperature.

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The equation used for the melting temperature is: Tm = 81.5 + 0.41(%GC) - 675/N - % mismatch, where N = total number of bases. Paste your primer sequence below ...

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Design PCR and qPCR assays intelligently for any species, with PrimerQuest. Fine-tune primer and probe design by adjusting the tool's custom design ...